TZ 5mg

TZ is a synthetic 39-amino acid peptide engineered from the native GIP sequence with incorporated GLP-1 receptor activity. It is the first dual glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) receptor agonist — a class referred to in the literature as a “twincretin.”

The compound features two non-coded α-aminoisobutyric acid (Aib) residues at positions 2 and 13 that confer resistance to DPP-4 enzymatic degradation, and a C20 fatty diacid moiety conjugated to Lys20 via a γGlu-2×OEG linker, enabling extended plasma half-life through albumin binding (~5 days). The C-terminus is amidated.

Research Applications

• Dual incretin receptor signaling and downstream pathway analysis (GIP/GLP-1)
• In-vitro models of glucose-dependent insulin secretion and glucagon suppression
• Metabolic pathway research involving lipid metabolism and energy homeostasis
• Receptor binding affinity and selectivity studies (GIPR vs. GLP-1R)
• Structural biology and cryo-EM investigations of peptide–receptor complexes

Published Research Context

Tirzepatide has been the subject of extensive preclinical and structural research. Cryo-EM studies (Sun et al., 2022) have resolved the peptide bound to both GLP-1R and GIPR in complex with G-proteins, revealing distinct interaction networks including ionic bonds, hydrogen bonding, and hydrophobic contacts at the receptor interfaces. The compound’s 14 unique amino acid substitutions (relative to native GLP-1) and its dual receptor engagement mechanism make it a significant tool for studying multiplexed pharmacological signaling.

Specifications

• Molecular Formula: C₂₂₅H₃₄₈N₄₈O₆₈
• Molecular Weight: 4,813.53 g/mol
• Sequence Length: 39 amino acids
• CAS Number: 2023788-19-2
• Form: White lyophilized powder
• Storage: −20°C lyophilized, protected from light; 2–8°C reconstituted
• Peplix Lot Purity: 99.95% by HPLC (Lot 2603)
• Minimum Purity: ≥98% by HPLC
• Testing Laboratory: Freedom Diagnostics

⚠ For in-vitro research use only. Not for human consumption. Not FDA approved. Certificate of Analysis included with every order.

📄 Documentation: Certificate of Analysis (Lot 2603)  |  Tirzepatide Compound Reference & Research Data  |  Reconstitution Calculator